The paper presents the results of experiments on the influence of the organic matter’s characteristics on the formation potential of water chlorination by-products – representatives of the following groups: trihalomethanes, haloacetonitriles, haloketones, chloral hydrate and chloropicrin. The products of water fractionation (the hydrophobic and hydrophilic acids, hydrophobic and hydrophilic bases, and hydrophobic and hydrophilic neutral fractions) were chlorinated with sodium hypochlorite. Its dose was adjusted to obtain a residual free chlorine concentration between 3 and 5 mg/dm3 after 24 h. After this time, the water chlorination by-products were analyzed with gas chromatography. The results’ analysis has defined the fractions, which have the highest potential to form particular groups of volatile organic water chlorination by-products.
Foam fractionation process for concentration of laccases from two Basidiomycete strains under different process conditions was investigated. Culture supernatants of Cerrena unicolor and Pleurotus sapidus containing active laccase were used with and without surfactant additives. Two surfactants: cationic cetrimonium bromide (CTAB) and non-ionic Polysorbate 80 were applied in the range from 0.2 mM to 1.5 mM. The pH levels ranging from 3 to 10 were examined with particular attention to pH=4, which is close to the pI of the enzymes. Results show that the source of the enzyme is significant in terms of partitioning efficiency in a foam fractionation process. Laccase from Cerrena unicolor showed the best activity partitioning coefficients between foamate and retentate of almost 200 with yields reaching 50% for pH 7.5 and concentration of CTAB cCTAB = 0.5 mM, whereas laccase from Pleurotus sapidus showed partitioning coefficients of up to 8 with 25% yield for pH 4 and cCTAB = 0.5 mM.
The research aim was to determine the long-term impact of the mine waste stored at the coal waste dump Hałda Ruda on the content of heavy metals in the bottom sediments of the Bytomka River. It is a watercourse flowing along this coal waste dump and has been under its influence for over fifty years. The research also attempted to determine the seasonality of changes (2 years) and mobility of selected elements. The article presents total contents of Cr, Mn, Ni, Cu, Zn, As, Cd and Pb in the bottom sediments collected from the Bytomka River. It also focuses on the distribution of these elements in particular geochemical fractions determined with the Tessier's sequential chemical extraction procedure. Total element contents were determined with an EDPXRF (Energy Dispersive X-ray Fluorescence) technique. The extractants of particular Tessier's fractions were determined quantitatively with an ICP-MS (Inductively Coupled Plasma Mass Spectrometry) spectrometer. The research results show that the stored waste significantly influences the contents of heavy metals in the Bytomka River bottom sediments. The lowest concentration of heavy metals was observed at the B1 spot (above the dump), while the highest one was measured at the B3 spot (below the dump). Sequential chemical extraction of the bottom sediments indicates that the Zn content in the ion-exchange and carbonate fractions diminished within a year. Nevertheless, Zn bound to Fe and Mn oxides acted in the opposite way. Mn, Zn and Pb are the most dangerous elements from the viewpoint of environmental hazards, as their total concentrations were high. Moreover, their high contents were observed in the most mobile (ion-exchange and carbonate) fractions. Extremely toxic Cd was bound to the oxide fraction to the largest extent. Cu was mainly bound to the organic fraction while environmentally hazardous Cr was bound to the residual fraction.
Antarctic krill carbohydrate content was followed during 1983—84 Eighth Polish Antarctic Expedition. The Admiralty Bay (King George Island) was th area of study. The following average values of three estimated fractions were obtained: 3.77 +- 1.51%, 0.47 +- 0.34% and 3.30 +- 1.33% for total, TCA-soluble and TCA-insoluble carbohydrates, respectively. Percentage contribution of the estimated fractions to dry weight varied seasonally (1.48—7.41%, 0.15—1.83%, and 1.28—6.28%, respectively). The carbohydrate content showed a clearcut cycle of changes over the calender year, with a minimum in autumn-winter and a maximum in spring-summer.
Changes in the amount of basic nitrogen fractions (total, protein and non-protein nitrogen) were studied in an annual cycle. Significant seasonal changes were noted, minima occurring in Antarctic winter and maxima during spring-summer season. These changes are due mainly to high fluctuations of water content in krill in the annual cycle.
In our recent study we demonstrated that the holding of fresh semen in fractionated seminal plasma (SP1, >40 kDa; SP2, <40 kDa), obtained by gel filtration chromatography, significantly improved the sperm quality characteristics following cryopreservation (Wasilewska-Sakowska et al. 2019). In this study we investigated the effect of post-thaw (PT) supplementation of fractionated SP (SP1 and SP2) on the survival of spermatozoa from boars with good and poor semen freezability, GSF and PSF, respectively. Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) analysis showed distinct differences in the protein profiles of SP1 and SP2 from boars with GSF or PSF regarding the number of protein spots. Sperm motility characteristics and the motion patterns, assessed using the computer-assisted sperm analysis (CASA) system, were markedly higher in PT semen supplemented with SP1 and SP2 from boars with GSF. Post-thaw supplementation of either SP1 or SP2 from boars with GSF significantly improved mitochondrial function, plasma membrane and acrosome integrity, and viability during storage. The findings of this study have confirmed that the presence of protective protein components in varying abundance in either fractionated SP from boars with good freezability ejaculates significantly improved the sperm survival following PT storage.
The stability analysis for discrete-time fractional linear systems with delays is presented. The state-space model with a time shift in the difference is considered. Necessary and sufficient conditions for practical stability and for asymptotic stability have been established. The systems with only one matrix occurring in the state equation at a delayed moment have been also considered. In this case analytical conditions for asymptotic stability have been given. Moreover parametric descriptions of the boundary of practical stability and asymptotic stability regions have been presented.
The article focuses on the fractional-order backward difference, sum, linear time-invariant equation analysis, and difficulties of the fractional calculus microcontroller implementation with regard to designing a fractional-order proportional integral derivative (FOPID) controller. In opposite to the classic proportional integral derivative (PID), the FOPID controller is defined by five independent parameters. Hence, it is more customizable and, potentially, more precise on condition that the values of fractional integration and differentiation orders are properly selected. However, a number of operations and the time required to calculate the output signal continuously increase. This can be a significant problem considering the limitations of a microcontroller, including memory size and a constant sampling time of the set-up analog-to-digital (ADC) converters. In the article, three solutions are considered, and results obtained in the experiments are presented.
The problem of of the use of fly ash still constitutes a research and exploration area for scientists. This is due to the fact that, 6,000,000 Mg of coal combustion by-products (CCB) are storage on landfills yearly in Poland alone. One of the potential directions of using fly ash is to use it as a substrate in hydrothermal syntheses of mesoporous materials (synthetic zeolites). Zeolites are aluminosilicates with a spatial structure. Due to their specific structure they are characterized by a number of specific properties among others molecular-sieve, ion-exchange and catalytic that can be used in engineering and environmental protection. So far, the synthesis has been carried out using coal combustion by-products such as fly ash or microsphere. The article analyzes whether separation from the fly ash of the appropriate fraction (below 63 μm) will affect the formation of zeolite grains. The syntheses were carried out using class F fly ash and the fraction separated from it, which was obtained by sieving the ash through a 63 μm sieve. Chemical (XRF) and mineralogical (XRD, SEM-EDS) analyzes were carried out for substrates as well as the obtained reaction products. In the case of substrates, the analysis did not show any significant differences between the ash and the separated fraction. However, in products after synthesis (Na-X zeolite with a small amount of Na-P1 zeolite, and small amounts of quartz and unreacted aluminosilicate glass - mullite) higher aluminum and sodium contents were observed from the separated fraction, with a lower calcium and potassium content. A small proportion of illite was observed on the diffraction curve of the zeolite from the fraction. Observations of grain morphology showed no differences in formation. Based on the conducted analyzes, it can be stated that, considering the economics of the synthesis process, the separation of fine fractions from the fly ash does not affect the quality of the synthesis process.
The paper discusses the modelling of magnetic coupling in ignition coils by fractional differential equations. The use of fractional-order coupling allows us to consider the losses caused by the non-linearity of the ferromagnetic core of the ignition coil and obtain the waveform of the ignition coil’s secondary voltage closest to the values obtained experimentally.
Electrophoretic methods were used to identify protein complexes formed between ostrich egg yolk lipoprotein fractions (LPFo) with seminal plasma (SP) of fractionated ejaculates, and to investigate the effect of these complexes on boar semen quality after cryopreservation. Chromatographic SP fractions (F1, F2 and F3), with or without LPFo solution, were subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis. Comparative electrophoretic analyses of the SP revealed marked differences in the SDS-PAGE protein profiles among boars. Electrophoretic analyses showed that the interactions of LPFo with SP resulted in the appearance of high-intensity protein bands. Spermatozoa were exposed to SP chromatographic fractions originating from F1, F2 and F3, and the whole SP (wSP) before being frozen. Spermatozoa exposed to F1 and F2 exhibited significantly higher post-thaw motility compared to those treated with either F3 or wSP. In most of the boars the proportions of membrane-intact frozen-thawed spermatozoa differed among the treatments, being significantly lower in the wSP-treated samples. The incidence of frozen-thawed spermatozoa with DNA fragmentation was less prevalent in samples exposed to F3 or the wSP. The results of this study confirmed that the interactions of LPFo with fractionated SP during the cooling period contributed to alterations in the sperm membranes, rendering them less susceptible to temperature-related injury.
Work is being carried out on possibilities of limiting the content of mercury in hard coal products by gravity concentration of run-of-mine coal in the Branch of the Institute of Mechanized Construction and Rock Mining in Katowice and on the Faculty of Energy and Fuels of the AGH University of Science and Technology in Krakow. Under domestic industrial conditions, gravity concentration is carried out with heavy medium liquids and in jigs. Preliminary - pilot studies have shown the possibility of mercury removal also by using the dry deshaling method involving vibratory air separators. Mercury is mainly found in the pyrite and the rubble formed by the mineral carbon, but also in the organic carbon. Some of it is located in layers of coal roof fields, which in the course of their exploitation go to coal. The mercury removal efficiency during the gravity concentration process will depend on the decomposition of the listed components in the density fractions. The paper presents the results of investigations of total mercury and total sulphur content in the separated coal fractions from four mines. These contents were determined in fractions: –1.5 g/cm3 (conventionally clean coal – concentrate), 1.5–1.8 g/cm3 (conventionally middlings) and +1.8 g/cm3 (conventionally rock – waste). The results are summarized in Tables 3–5 and in Charts 1–4. Conversely, graphs 5-8 show the relationship between mercury content and total sulphur content in the tested coal samples. The study, which can be called a preliminary analysis of the susceptibility of the coals to gravity concentration, showed that the dry deshaling method on the vibratory air separators would allow significant amounts of mercury accumulated in the middlings and waste fractions to be removed.