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Abstract

Metal ions can modify plant metabolism and change the level of biologically active components. In the present study, the impact of short-term exposure to strontium on the accumulation of the metal as well as the content of isoflavones in soybean sprouts was investigated. The seeds were germinated in hydroponics with 0, 1, 1.5, 2.5, 5.0, or 10.0 mM of Sr for 72 hours. The content of strontium was assessed using flame atomic absorption spectrometry and the amount of isoflavones was determined with high performance liquid chromatography. Dose-dependent accumulation of Sr and a linear correlation between the Sr concentration in the growth medium and the content of the element in the plant samples were observed. The largest changes in the isoflavone content, compared to the control, were noted in soy sprouts germinated in the presence of 5 and 10 mM of strontium. Daidzin, genistin, malonyldaidzin, and malonylgenistin were the dominant isoflavones and their content increased by approx. 28, 44, 34, and 47%, respectively, compared to the control. Low amounts of aglycones were found; moreover, their content decreased by ca. 19–30%. Our research can be important for obtaining a natural product enhanced with strontium and isoflavones, which contribute to prevention of osteoporosis associated with endogenous oestrogen deficits.
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Abstract

The “second generation” of glyphosate-tolerant soybean (GT2 soybean) was developed through a different technique of insertion of the glyphosate-insensitive EPSPs gene, in comparison with “first generation” of glyphosate-tolerant soybean. However, there is not enough information available about glyphosate selectivity in GT2 soybean and the effects on the quality of seeds produced. The aim of this study was to evaluate tolerance to glyphosate and seed quality of soybean cultivar NS 6700 IPRO (GT2) with cp4-EPSPs and cry1Ac genes, after application at post-emergence (V4). The experiment was conducted in a randomized block design with four replicates and seven treatments, or rates of glyphosate (0; 720; 1,440; 2,160; 2,880; 3,600; 4,320 g of acid equivalent − a.e. · ha−1). Assessments were performed for crop injury, SPAD index and variables related to agronomic performance and seed quality. A complementary trial with the same cultivar and treatments in a greenhouse was conducted in a completely randomized design with four replications. Data analysis indicated no significant effect of glyphosate on V4 on agronomic performance and physiological quality of seeds, for two growing seasons. The soybean cultivar NS 6700 IPRO (GT2), with cp4-EPSPs and cry1Ac genes, was tolerant to glyphosate up to the maximum rate applied (4,320 g a.e. · ha−1) at post-emergence (V4). The quality of soybean seeds was not affected by glyphosate up to the maximum rate applied (4,320 g a.e. · ha−1) at post-emergence (V4).
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Abstract

The aim of the present work was to evaluate the selectivity of nicosulfuron, alone and in combinations, applied in post-emergence (V4) of glyphosate and sulfonylurea tolerant (RR/STS) soybean. The experiments were conducted in 2015/16 and 2016/17, in Piracicaba – state of São Paulo (SP). In 2016/17, the experiment was also conducted in Palotina – state of Paraná (PR). The experiment was a randomized block design, with four repetitions and 16 treatments, with combinations of nicosulfuron, glyphosate, chlorimuron, sulfometuron and cloransulam, applied alone or in tank mixture. Crop injury and variables related to agronomic performance were evaluated. Data were subjected to analysis of variance and treatment means were compared by the Tukey test. The results obtained are significant in the positioning of herbicides in RR/STS soybean, since in the five experiments, all the treatments were selective, except for glyphosate + sulfometuron which reduced the yield of a cultivar (CD 2630 RR/STS) in the 2015/16 season.
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