The primary evaluation of the economic losses caused by water pollution in Shanghai in the year 2009 is made by classification approach in order to provide basis for decision of the relative water management policy. The result shows that the portion of water pollution losses in GDP of Shanghai was 2.7%, which was still lower than the average level of whole China despite of the local high population density and the scale of industry, suggesting to some extent the continuous attention in water protection paid by Shanghai government.
Heterogeneous nuclear ribonucleoprotein K (hnRNP K), is a multifunctional protein that participates in a variety of regulatory processes of signal transduction and gene expression. To further characterize the significance of hnRNP K in different male germ cells, we investigated the expression profiles of hnRNP K at different developmental stages in pig and rat testes, and conducted a comparative analysis of expression patterns between these two species. In porcine testis development, both the mRNA and protein level of hnRNP K were down-regulated from 3 months to 8 months. However, the expression level of hnRNP K was abundant across the embryonic period in rats, and decreased gradually from 0 day post partum (dpp) to 14 dpp, then increased with the highest level presenting at 90 dpp. Immunolocalization analysis further confirmed the differential expression and localization of hnRNP K protein during testis development in pigs and rats. The results showed that hnRNP K was widely distributed in gonocytes, spermatogonia, sertoli cells and Leydig cells. The dynamic expression profile of hnRNP K may imply its crucial and potential roles in the development of the testis, which will provide a theoretical basis for the future study of molecular mechanism regulation of spermatogenesis.
A mixed pseudo-orthogonal frequency coding (Mixed-POFC) structure is proposed as a new spreadspectrum technique in this paper, which employs frequency and time diversity to enhance tag properties and balances the spectrum utilization and code diversity. The coding method of SAW RFID tags in this paper uses Mixed-POFC with multi-track chip arrangements. The cross-correlation and auto correlation of Mixed-POFC and POFC are calculated to demonstrate the reduced overlap between the adjacent center frequencies with the Mixed-POFC method. The center frequency of the IDT and Bragg reflectors is calculated by a coupling of modes (COM) module. The combination of the calculation results of the Bragg reflectors shows that compared with a 7-chip POFC, the coding number of a 7-chip Mixed-POFC is increased from 120 to 144 with the same fractional bandwidth of 12%. To demonstrate the validity of Mixed-POFC, finite element analysis (FEA) technology is used to analyze the frequency characteristics of Mixed-POFC chips. The maximum error between designed frequencies and simulation frequencies is only 1.7%, which verifies that the Mixed-POFC method is feasible.
With the increasing penetration rate of grid-connected renewable energy generation, the problem of grid voltage excursion becomes an important issue that needs to be solved urgently. As a new type of voltage regulation control method, electric spring (ES) can alleviate the fluctuations of renewable energy output effectively. In this paper, the background and basic principle of the electric spring are introduced firstly. Then, considering the influence of an electric spring on noncritical load voltage, noncritical loads are classified reasonably, and based on the electric spring phasor diagram, the control method to meet the noncritical load voltage constraint is proposed. This control method can meet the requirements of voltage excursions of different kinds of noncritical load, increase the connection capacity of the noncritical load and improve the voltage stabilization capacity of the electric spring. Finally, through the simulation case, the feasibility and validity of electric spring theory and the proposed control method are verified.
In this study, medium-carbon steel was subjected to warm deformation experiments on a Gleeble 3500 thermosimulator machine at temperatures of 550°C and 650°C and strain rates of 0.001 s–1 to 1 s–1. The warm deformation behavior of martensite and the effects of strain rate on the microstructure of ultrafine grained medium-carbon steel were investigated. The precipitation behavior of Fe3C during deformation was analyzed and the results showed that recrystallization occurred at a low strain rate. The average ultrafine ferrite grains of 500 ± 58 nm were fabricated at 550°C and a strain rate of 0.001 s–1. In addition, the size of Fe3C particles in the ferrite grains did not show any apparent change, while that of the Fe3C particles at the grain boundaries was mainly affected by the deformation temperature. The size of Fe3C particles increased with the increasing deformation temperature, while the strain rate had no significant effect on Fe3C particles. Moreover, the grain size of recrystallized ferrite decreased with an increase in the strain rate. The effects of the strain rate on the grain size of recrystallized ferrite depended on the deformation temperature and the strain rate had a prominent effect on the grain size at 550°C deformation temperature. Finally, the deformation resistance apparently decreased at 550°C and strain rate of 1 s–1 due to the maximum adiabatic heating in the material.
Recycling construction and demolition waste not only reduces project costs; and saves natural resources, but also solves the environmental threat caused by construction waste disposal. In this paper, C25 waste road concrete is used as an experimental material, the uniaxial compression strength and tensile splitting strength of C25 RAC whose coarse aggregate replacement rate is 0%, 25%, 50%, 75%, and 100% are tested under the condition that the water-to-cement ratio is 0.47, 0.55 and 0.61. The results show: (1) the uniaxial compression strength and tensile splitting strength decrease with the increase of RAC; (2) for concrete with the same water-to-cement ratio, when the coarse aggregate replacement rate changes from 0% to 50%, the uniaxial compression strength and tensile splitting strength of RAC changes slightly. When the coarse aggregate replacement rate changes from 50% to 100%, the uniaxial compression strength and tensile splitting strength of RAC decreases rapidly
Reliable estimation of longitudinal force and sideslip angle is essential for vehicle stability and active safety control. This paper presents a novel longitudinal force and sideslip angle estimation method for four-wheel independent-drive electric vehicles in which the cascaded multi-Kalman filters are applied. Also, a modified tire model is proposed to improve the accuracy and reliability of sideslip angle estimation. In the design of longitudinal force observer, considering that the longitudinal force is the unknown input of the electric driving wheel model, an expanded electric driving wheel model is presented and the longitudinal force is obtained by a strong tracking filter. Based on the longitudinal force observer, taking into consideration uncertain interferences of the vehicle dynamic model, a sideslip angle estimation method is designed using the robust Kalman filter and a novel modified tire model is proposed to correct the original tire model using the estimation results of longitudinal tire forces. Simulations and experiments were carried out, and effectiveness of the proposed estimation method was verified.
The heavy metal release experiments were conducted in the laboratory to examine the effects of 3 factors - pH, dissolved oxygen (DO), and temperature on the metal release from sediments taken from the Huangpu River. The metal concentrations in the dry sediments ranged from 0.030 to 0.296 mg g-1 for Cr, 0.021 to 0.097 mg g-1 for Ni, 0.014 to 0.219 mg g-1 for Cu, 0.035 mg to 0.521 mg g-1 for Zn, 0.0002 to 0.001 mg g-1 for Cd and 0.023 to 0.089 mg g-1 for Pb. Most of the metals found in the sediments were in the form of residual fraction, the exchangeable fraction consisted of only a small portion of total metals. The average dissolved metal concentrations in the overlying water during the 13-day period under different conditions were ranging from 0.82 to 1.93 μg L-1 for Cr, 1.08 to 4.19 μg L-1 for Ni, 40.79 to 82.28 μg L-1 for Cu, 20.30 to 29.96 μg L-1 for Zn, 1.57 to 4.07 μg L-1 for Cd, and 22.26 to 75.50 μg L-1 for Pb, respectively. Statistical interpretation of the data indicated that pH (7, 8, 9), dissolved oxygen DO (1.0 and 5.0 mg L-1) and temperature (4, 16, 25°C) had no significant effects on the heavy metal release under the studied conditions. Cu and Pb had the highest release flux, while Cd, Pb and Cu had higher mobility. The main factors controlling the metals release might be the inherent characters of metals and sediments.
In vitro embryogenic callus is a critical factor for genetic transformation of rice, especially for indica varieties. In this study, we investigated the relationship between polyamines, including putrescine (Put), spermidine (Spd) and spermine (Spm), and callus browning, and we studied the effect of exogenous Put on callus regeneration and on the content of endogenous polyamines. In addition, the expression levels of arginine decarboxylase gene (Adcl) and S-adenosylmethionine decarboxylase gene (Samdc) in embryogenic callus were studied by quantitative Real-time PCR analysis. The results showed that the contents of endogenous Put and Spd in the browning callus were significantly lower than those in normal callus. Exogenous Put could effectively improve the growing state of callus of indica rice and enhance the development of embryogenic callus. The content of endogenous polyamines in embryogenic callus, especially Spd and Spm, was increased after addition of exogenous Put. Additionally, exogenous Put also had an obvious impact on the expression levels of Adcl but partial effect on the expression levels of Samdc gene. This study could increase the knowledge of both embryogenic callus induction and polyamine catabolism in callus in indica rice.
The effects of Mg and Ca on sulfide modification of sulphur steel were studied to elucidate the difference between micromagnesium treatment and micro-calcium treatment for the inclusion of sulphur steel. The results show that the inclusions in the steel appeared with an oxide core of Al2O3 and MnS wrapped. After the addition of Mg, the core was changed to spinel, and the MnS coating was changed to Mn-Mg-S. After Ca was added, the core was changed to Ca-Al-O, and the MnS coating was changed to Mn-Ca-S. The Mg content was higher than Ca content in the sulfides of the steel. Therefore, Mg was more effective than Ca in terms of sulfide modification with the same content of Mg and Ca in steel, but the yielding rate of Mg was lower than that of Ca. The Mg content in the oxide core was higher than Mg of the coating of the inclusions in the steel treated with Mg or Mg-Ca. In contrast, the Ca content in the oxide core was lower than Ca of the coating of the inclusions in the steel treated with Ca or Mg-Ca. MnS formed and precipitated during the melt solidification process. The complex sulfide (Mg-Mn-S) was precipitated around MgO·Al2O3 in the Mg treated steel during the cooling process. CaS inclusion was precipitated on the CaO·Al2O3 inclusions in the liquid Ca-treated steel. Thus, CaS was formed first, whereas MnS was formed during the cooling process, followed by the formation of complex sulfide (CaS+MnS), which finally precipitated around CaO·Al2O3 in the Ca-treated steel.
Sound joint of hollow-extruded 6005A-T6 aluminum alloy was achieved by friction stir welding and its high cycle fatigue performance was mainly investigated. As a result, the joint fatigue limit reaches 128.1 MPa which is 55% of the joint tensile strength. The fatigue fracture mainly occurs at the boundary between the stir zone and thermo-mechanically affected zone due to the large difference in the grain size. This difference is caused by the layered microstructure of the base material. The shell pattern with parallel arcs is the typical morphology in the fracture surface and the distance between arcs is increased with the increase of stress level. The specimen with the fracture located in the stir zone possesses a relatively low fatigue life.