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Abstract

Spare parts are one of the important pillars in the after-sales service of automotive business. Customers will satisfied and comfortable if the availability of spare parts is guaranteed. Spare Part Center is one of function to support unit sales and as well as profit-oriented, so the accuracy and speed of spare part acceptance by the customer is an important key to winning the competition. Order Picking is one of the supply chain processes that play a role in warehouse operations to meet customer needs. Order Picking is the most expensive activity in warehousing and can reach 55% of the total cost of warehousing operations, so it is considered a top priority in increasing productivity, even reaching 65% of total warehouse operating costs. The purpose of this research is to increase productivity in the process of picking order through reduction of processing time. Increased productivity is done by improving the working method of the picking process. From the result the comparing, the method by zone requires less total picking time (193.712 seconds) than by routing (249.559 seconds) decreased 55.85 second time, in other words, an increase of 22.38%. With the Visual Stream Mapping (VSM) in this research can reduce to travel time, it means that the total distance traveled is small than the current method. The impact from VSM approach will eliminate time for preparation of 1.960 seconds, and take empty trolley of 200 seconds. In this case some of traveling non-value
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Abstract

We assessed culturable soil microfungal diversity in various habitats around Hornsund, Spitsbergen in the High Arctic, using potato dextrose agar ( PDA) medium. Thermal growth classification of the fungi obtained was determined by incubating them in 4 ° Cand 25 ° C, permitting separation of those with psychrophilic, psychrotolerant and mesophilic characteristics. In total, 68 fungal isolates were obtained from 12 soil samples, and grouped into 38 mycelial morphotypes. Intergenic spacer regions of these morphotypes were sequenced, and they represented 25 distinct taxonomic units, of which 21 showed sufficient similarity with available sequence data in NCBI to be identified to species level. Soil under ornithogenic influence showed the highest species diversity, including sequences assigned to Mortierella macrocystis, M. elongata, Mortierella sp., Cudoniella sp., Varicosporium elodeae , Beauveria bassiana , Geomyces pannorum , Penicillium sp. and Atradidymella muscivora . Fourteen taxa were classified as psychrophilic, seven mesophilic, and four psychrotolerant.
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Abstract

This report describes the isolation and characterization of bacterial isolates that produce anti−microbial compounds from one of the South Shetland Islands, King George Is − land, Antarctica. Of a total 2465 bacterial isolates recovered from the soil samples, six (BG5, MTC3, WEK1, WEA1, MA2 and CG21) demonstrated inhibitory effects on the growth of one or more Gram−negative or Gram−positive indicator foodborne pathogens ( i.e. Escherichia coli 0157:H7, Salmonella spp., Klebsiella pneumoniae , Enterobacter cloacae , Vibrio parahaemolyticus and Bacillus cereus ). Upon examination of their 16S rRNA sequences and biochemical profiles, the six Antarctic bacterial isolates were identified as Gram−negative Pedobacter cryoconitis (BG5), Pseudomonas migulae (WEK1), P. corrugata (WEA1) and Pseudomonas spp. (MTC3, MA2, and CG21). While inhibitors produced by strains BG5, MTC3 and CG21 were sensitive to protease treatment, those produced by strains WEK1, WEA1, and MA2 were insensitive to catalase, lipase, a −amylase, and protease enzymes. In addtion, the six Antarctic bacterial isolates appeared to be resistant to multiple antibiotics.
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Abstract

A filamentous benthic cyanobacteria, strain USMAC16, was isolated from the High Arctic Svalbard archipelago, Norway, and a combination of morphological, ultrastructural and molecular characterisation (16S rRNA gene sequence) used to identify to species level. Cell dimensions, thylakoid arrangement and apical cell shape are consistent with the Pseudanabaena genus description. The molecular characterisation of P. catenata gave 100% similarity with Pseudanabaena catenata SAG 1464-1, originally reported from Germany. Strain USMAC16 was cultured under a range of temperature and photoperiod conditions, in solid and liquid media, and harvested at exponential phase to examine its phenotypic plasticity. Under different culture conditions, we observed considerable variations in cell dimensions. The longest cell (5.91±0.13 μm) was observed at 15°C under 12:12 light:dark, and the widest cell (3.24±0.06 μm) at 4°C under 12:12 light: dark in liquid media. The study provides baseline data documenting the morphological variation of P. catenata in response to changing temperature regimes.
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