In order to calibrate the stripe precision of a leveling rod, an automatic laser interferometer and a vision measurement system were designed by Xi’an University of Technology in China. The rod was driven by a closed-loop control and the data were collected at the stop state to ensure precision. The laser interferometer provided not only the long distance data but also a position feedback signal in the automatic control loop. CCD camera and a vision measurement method were used to inspect the stripe edge position and defect. A pixel-equivalent self-calibration method was designed to improve precision. ROI (regions of interest) method and an outline tracing method were designed to quickly extract multiple stripe edges. A combination of the image data with the interferometer data reduces control difficulty and ensures the measurement accuracy. The vision measurement method reached sub-pixel precision and the defective edges were reported. The system can automatically calibrate a stripe leveling rod with a high degree of efficiency and precision.