The reliable and rapid diagnosis of infectious animal diseases presents an exceptionally im- portant aspect when considering their control and prevention. The paper describes the compara- tive evaluation of two rapid isothermal amplification methods for diagnosis of African swine fever (ASF). The robustness of loop-mediated isothermal amplification (LAMP) and the cross-priming amplification (CPA) were compared using samples obtained from ASF confirmed animals. Both assays were evaluated in order to define their diagnostic capabilities in terms of ASF diagnosis and reproducibility of the results. Investigations showed no cross-reactivity for other pig patho- gens and no significant differences in the specificity of both assays. The sensitivity of LAMP reached 90%, while that of CPA was 70%. In conclusion, both methods are suitable for imple- mentation in preliminary ASF diagnosis but further improvements are required to enhance their diagnostic sensitivity.
Simulation software can be used not only for checking the correctness of a particular design but also for finding rules which could be used in majority of future designs. In the present work the recommendations for optimal distance between a side feeder and a casting wall were formulated. The shrinkage problems with application of side feeders may arise from overheating of the moulding sand layer between casting wall and the feeder in case the neck is too short as well as formation of a hot spot at the junction of the neck and the casting. A large number of simulations using commercial software were carried out, in which the main independent variables were: the feeder’s neck length, type and geometry of the feeder, as well as geometry and material of the casting. It was found that the shrinkage defects do not appear for tubular castings, whereas for flat walled castings the neck length and the feeders’ geometry are important parameters to be set properly in order to avoid the shrinkage defects. The rules for optimal lengths were found using the Rough Sets Theory approach, separately for traditional and exothermic feeders.