Globally more than 5.2 billion hectares of farming fields are damaged through erosion, salinity and soil deterioration. Many salt stress tolerant bacteria have plant growth promoting (PGP) characteristics that can be used to overcome environmental stresses. Isolation and screening of salt-tolerant endophytes from Salicornia brachiata were achieved through surface sterilization of leaves followed by cultivation on 4% NaCl amended media. Performance of isolates towards indole-3-acetic acid (IAA) production, phosphate solubilization, ACC deaminase activity, ammonia production, siderophore production and stress tolerance were determined. On the basis of the highest plant growth promoting activity, SbCT4 and SbCT7 isolates were tested for plant growth promotion with wheat and maize crops. In the present study, a total of 12 morphologically distinct salt-tolerant endophytic bacteria was cultured. Out of 12 isolates, 42% of salt-tolerant endophytes showed phosphate solubilization, 67% IAA production, 33% ACC-deaminase activity, 92% siderophore production, 41.6% ammonia production and 66% HCN production. A dendrogram, generated on the basis of stress tolerance, showed two clusters, each including five isolates. The bacterial isolates SbCT4 and SbCT7 showed the highest stress tolerance, and stood separately as an independent branch. Bacterial isolates increased wheat shoot and root dry weights by 60–82% and 50–100%, respectively. Similarly, improved results were obtained with maize shoot (27–150%) and root (80–126%) dry weights. For the first time from this plant the bacterial isolates were identified as Paenibacillus polymyxa SbCT4 and Bacillus subtilis SbCT7 based on phenotypic features and 16S rRNA gene sequencing. Paenibacillus polymyxa SbCT4 and B. subtilis SbCT7 significantly improved plant growth compared to non-inoculated trials.
O b j e c t i v e s: The aim of study was to investigate the association between anxiety, depression, stress and determinants of quality of life among Iranian students. M e t h o d s: The questionnaires were completed by 275 students. The random sampling was conducted in two phases, the stratified sampling which some classes were selected among different classes of faculty of health and at the second phase, in each class the number of students who had the requirements to enter in the study were selected randomly. the logistic regression to find out the association between demographic characteristics with the quality of life was run and according to the normality status of the distribution of data the parametric or non-parametric tests were used. R e s u l t s: In the univariable model, the students that were living in their own homes had the odds of 2.18 times more than the others to have a higher quality of life level (95% CI: 1.07–4.45). In the multi variable model the anxiety and stress were significantly related to the quality of life and for increasing each 1 unit in the amount of anxiety and stress the odds of a better quality of life decreases 0.19 and 0.03 respectively. Even after adjusting for other covariates – in the multivariable model – both anxiety and stress were associated with the quality of life. C o n c l u s i o n: It is useful for the universities to understand different aspects of the students’ lives which are under the influence of stress, anxiety and depression, and also determining the resources from which they are originated.
Microbes living in the polar regions have some common and unique strategies to respond to thermal stress. Nevertheless, the amount of information available, especially at the molecular level is lacking for some organisms such as Antarctic psychrophilic yeast. For instance, it is not known whether molecular chaperones in Antarctic yeasts play similar roles to those from mesophilic yeasts when they are exposed to heat stress. Therefore, this project aimed to determine the gene expression patterns and roles of molecular chaperones in Antarctic psychrophilic Glaciozyma antarctica PI12 that was exposed to heat stress. G. antarctica PI12 was grown at its optimal growth temperature of 12ºC and later exposed to heat stresses at 16ºC and 20ºC for 6 hours. Transcriptomes of those cells were extracted, sequenced and analyzed. Thirty-three molecular chaperone genes demonstrated differential expression of which 23 were up-regulated while 10 were down-regulated. Functions of up-regulated molecular chaperone genes were related to protein binding, response to a stimulus, chaperone binding, cellular response to stress, oxidation, and reduction, ATP binding, DNA-damage response and regulation for cellular protein metabolic process. On the other hand, functions of down-regulated molecular chaperone genes were related to chaperone-mediated protein complex assembly, transcription, cellular macromolecule metabolic process, regulation of cell growth and ribosome biogenesis. The findings provided information on how molecular chaperones work together in a complex network to protect the cells under heat stress. It also highlights the evolutionary conserved protective role of molecular chaperones in psychrophilic yeast, G. antarctica, and mesophilic yeast, Saccharomyces cerevisiae.
The phytotoxic effects of fluoride and its derivatives on the seeds and seedlings of the Colobanthus apetalus and Colobanthus quitensis were studied. This is a first study to evaluate the influence of sodium fluoride (NaF) on the morphophysiological and biochemical processes on two Colobanthus species. The influence of various concentrations of NaF (9 mM, 19 mM, 29 mM) on the germination capacity and germination rate of seeds, seedlings growth and the proline content of plant tissues was analyzed under laboratory conditions (20/10°C, 12/12 h). The seeds of C. apetalus were collected from a greenhouse, whereas the seeds of C. quitensis were collected in Antarctica and in a greenhouse (Olsztyn, Poland). The tested concentrations of NaF did not suppress the germination of C. apetalus seeds, but the germination of C. quitensis seeds was inhibited. Sodium fluoride mainly inhibited root growth of C. apetalus and C. quitensis. In both analyzed species, the free proline content of seedlings increased significantly under exposure to NaF. The results of this study clearly indicate that C. apetalus and C. quitensis are highly resistant to NaF stress.
The aim of the present study was to determine the concentrations of glutathione (GSH), vitamin C, copper (Cu) and zinc (Zn) in the uterine tissues in diagnosis of canine pyometra. Fourteen samples of uterine tissues from female dogs with pyometra and twelve samples of healthy uteruses (control) were used. The concentrations of GSH and vitamin C were determined in the uterine tissue homogenates using spectrophotometric methods. The concentrations of Cu and Zn were measured using atomic absorption spectrometer. The results obtained showed the significantly lower (p<0.05) concentration of GSH and the trend towards lower concentration of vitamin C in the pyometra samples compared to the control. The concentrations of Cu and Zn were similar in the uterine tissues from female dogs with pyometra and those from healthy female dogs. The lower GSH and vitamin C concentrations in the uterine tissues of female dogs with pyometra indicate that the non-enzymatic antioxidant mechanisms are impaired in the uterus of dogs with pyometra. These findings suggest that the imbalance of oxidative-antioxidative can play an important role in pathogenesis of canine pyometra.
An algorithm of determination of mechanical stresses and deformations of the magnetic circuit shape, caused by forces of magnetic origin, is presented in this paper. The mechanical stresses cause changes of magnetizing characteristics of the magnetic circuit. The mutual coupling of magnetic and mechanical fields was taken into account in the algorithm worked out. A computational experiment showed that it was possible to include the interaction of both fields into one numerical model. The elaborated algorithm, taking into account the impact of mechanical stresses on magnetic parameters of construction materials, can be used in both the 2D and the 3D type field-model.
Buckling of the stiffened flange of a thin-walled member is reduced to the buckling analysis of the cantilever plate, elastically restrained against rotation, with the free edge stiffener, which is susceptible to deflection.Longitudinal stress variation is taken into account using a linear function and a 2nd degree parabola. Deflection functions for the plate and the stiffener, adopted in the study, made it possible to model boundary conditions and different buckling modes at the occurrence of longitudinal stress variation. Graphs of buckling coefficients are determined for different load distributions as a function of the elastic restraint coefficient and geometric details of the stiffener. Exemplary buckling modes are presented.
This study was carried out on the background of Sutong Bridge project based on fracture mechanics, aiming at analyzing the growth mechanism of fatigue cracks of a bridge under the load of vehicles. Stress intensity factor (SIF) can be calculated by various methods. Three steel plates with different kinds of cracks were taken as the samples in this study. With the combination of finite element analysis software ABAQUS and the J integral method, SIF values of the samples were calculated. After that, the extended finite element method in the simulation of fatigue crack growth was introduced, and the simulation of crack growth paths under different external loads was analyzed. At last, we took a partial model from the Sutong Bridge and supposed its two dangerous parts already had fine cracks; then simulative vehicle load was added onto the U-rib to predict crack growth paths using the extended finite element method.
Incomplete oxygen reduction gives rise to reactive oxygen species (ROS). For a long time they have been considered unwelcome companions of aerobic metabolism. Organisms using oxygen developed several systems of ROS scavenging with enzymatic and non enzymatic antioxidants, which allow them control the cellular level of oxygen derived from free radicals. It is well established nowadays that ROS are not necessarily negative byproducts, but they also play an important role in cellular mechanisms. They are involved in many regular cellular processes in all aerobic organisms. When the antioxidant system is overcome and the balance between ROS production and scavenging is disrupted, oxidative stress occurs. It has been reported that oxidative stress may be linked to some human diseases and is also involved in biotic and abiotic stress response in plants.
Plants are continuously exposed to various environmental stresses and they respond to them in different ways. Ambient temperature is among the most important environmental cues that directly influence plant growth and yield. Research in recent years has revealed that epigenetic mechanisms play a key role in plants' response to temperature stress. Changes in gene expression evoked by stress signals follow post-translational histone modifications, DNA methylation, histone variant incorporation, and the action of chromatin remodeling factors and Polycomb group proteins. The majority of epigenetic modifications induced by temperature stress are reversible in nature; thus, chromatin returns to its previous state after the stress has passed. Some modifications seem stable, however, due presumably to so-called stress memory. Epigenetic modifications can be inherited through mitosis and meiosis. By dint of epigenetic memory, plants can more efficiently respond to future stressful conditions, thereby increasing their potential for environmental adaptation. Recognition of the epigenetic mechanisms that take part in plants' response to changes of ambient temperature will increase our understanding of adaptations to stress conditions.
Heavy metal pollution of soil is a significant environmental problem and has a negative impact on human health and agriculture. Phytoremediation can be an alternative environmental treatment technology, using the natural ability of plants to take up and accumulate pollutants or transform them. Proper development of plants in contaminated areas (e.g. heavy metals) requires them to generate the appropriate protective mechanisms against the toxic effects of these pollutants. This paper presents an overview of the physiological mechanisms of stress avoidance and tolerance by plants used in phytoremediation of heavy metals.
Many Antarctic marine benthic invertebrates are adapted to specific environ− mental conditions (e.g. low stable temperatures, high salinity and oxygen content). Changes caused by global climatic shifts can be expected to have significant impact on their physiol− ogy and distribution. Odontaster validus, an ubiquitous, omnivorous sea star is one of the “keystone species” in the Antarctic benthic communities. Laboratory experiments were car− ried out to study the effect of temperature rise (from 0 to 5#2;C) on some vital biological func− tions that sea stars must perform in order to survive in their environment. Parameters such as behavioural reaction of sea stars to food and food odour, locomotory performance and abil− ity to right were measured. Temperature increase significantly impaired the ability of O. validus to perform these functions (e.g. lowering the number of sea stars able to right, in− creasing time−to−right, reducing locomotory activity, weakening chemosensory reaction to food and food odour). At temperatures of 4 and 5#2;C a loss of motor coordination was ob− served, although at all tested temperatures up to 5#2;C there were single individuals perform− ing successfully.
Endopolyploidy is a condition of a cell containing reduplicated genetic material in its nucleus. Cells with the nuclei of different ploidy levels are often present within a single polysomatic organism. Endoreduplication is thus a modified cell cycle that omits cytokinesis and leads to chromatin replication in the endopolyploid cells. This study aimed to research the effect of salinity on endopolyploidy of Trifolium pratense and T. repens. Both species are important pasture legumes and belong to the genus Fabaceae with the well documented endopolyploidy occurence. Endopolyploidy levels in the seedlings treated with 0, 30, 60, 90 and 120 mM NaCl were investigated by flow cytometry. The seedling organs were evaluated during three ontogeny stages. The cytometric data plotted on a histogram showed the presence of 2C-16C nuclei in T. pratense and 2C-8C in T. repens. The hypothesis that salinity induces additional endocycles was not confirmed. Our results show that the distribution of nuclei among ploidy levels does not differ markedly between the treatment groups and the control ones. Additionally, only minor changes were observed among the endoreduplication indexes (EI) of plant organs after exposure to various salt concentrations. Endopolyploidy patterns within the salt-treated seedlings during ontogeny are similar to the controls. We suggest that endopolyploidy in Trifolium species is a conserved genetic trait, rather than an adaptation to salinity stress. The analyses of the roots of T'. pratense at stage III show that with the increased concentrations of NaCl the length of roots decreased, but no evident changes in endopolyploidy occured.
Purpose: The aim of this paper is to discuss exposure to stress and the incidence of occupational burnout among oncology nurses. Methods: To study the discussed issue, we analyzed six full-text research papers which were searchable by EBSCO and met all required criteria (words included in the abstract, English publication, size of the study group). Results: Exposure to chronic occupational stress may lead to developing burnout syndrome. Social service professionals are especially affected as they are expected to be emotionally engaged in their jobs, which particularly applies to such health care professionals as nurses, psychologists, police officers and social workers. Because of occupational burnout work efficiency may deteriorate. Oncology nurses are among the most affected nurse groups in terms of exposure to the risk of burnout. Conclusions: Oncology nurses as well as other oncology workers exhibit an increased risk and a higher grade of burnout. Psychological training sessions are available which effectively prevent and alleviate the effects of burnout.
The brake linkage of a hoisting machine is a very important component determining the safety of the hoisting machine’s entire braking system. It is subject to weekly inspections. However, an efficiency test of brake performance is carried out every 6 months. Once every 3 years, a test must be carried out by an appraiser who pays particular attention to the executive and control components of the brakes as well as the strain - brake system and brake release components. The legal provisions regulating the testing of braking system linkages are not precise. So far, the control has been based on random measurement of strains using electrical resistance strain gauges stuck to the surface of the linkage. A new method for measuring the strains of the linkage has been proposed in the work. It is based on fibre optic strain sensors with Fibre Bragg Gratings (FBG). They are mounted using specially designed and tested holders for mounting on the brake linkage. They provide quick assembly and the measurement of strain in the direction parallel to the axis of the linkage. The structure of the holder also allows for the measurement in 4 positions turned every 90 relative to one another. Such a measurement enables a comprehensive analysis of strains and stresses in the brake linkage. In the work, it was shown that there is a complex state of strain and stress in the brake linkage. The previous procedures for linkage testing are inadequate in relation to this condition. An experimental and numerical method was proposed to assess the state of linkage stress. It should constitute the basis for the decision of the appraiser to allow the linkage for further use. The method proposed in the work also allows for continuous measurements of linkage strains as well as dynamic braking tests.
The main aim of the study was to determine the goodness of fit between the relaxation function described with a rheological model and the real (experimental) relaxation curves obtained for digital materials fabricated with a Connex 350 printer using the PolyJet additive manufacturing technology. The study involved estimating the uncertainty of approximation of the parameters of the theoretical relaxation curve. The knowledge of digital materials is not yet sufficient; their properties are not so well-known as those of metallic alloys or plastics used as structural materials. Intensive research is thus required to find out more about their behavior in various conditions. From the calculation results, i.e. the uncertainty of approximation of the relaxation function parameters, it is evident that the experimental curves coincide with the curves obtained by means of the solid model when the approximation uncertainty is taken into account. This suggests that the assumed solid model is well-suited to describe a real material.
This paper presents the current study of the distribution of stresses for four-point contact wire race ball bearing. The main aim of this article is to define the most important geometrical rules in a wire-race bearing. The results for bearings of different geometrical parameters are presented. In the study, one also estimates the distribution of internal pressure in particular bearing elements.
The paper concerns simulation of fully developed and axially-symmetrical turbulent flow of coarse-dispersive slurry if all solid particles have similar size and shape with particles diameter from 1 mm to 5 mm, solid density from 1045 kg/m^3 to 3000 kg/m^3, and solid concentration by volume from 20% to 40%. The author examines the influence of particle diameter on additional shear stress due to the ‘particles-wall’ interactions for moderate and high solid concentration. The mathematical model was developed using Bagnold's concept,  and assumes that the total wall shear stresses are equal to the sum of ‘liquid-wall’ and ‘particles-wall’ shear stresses. The mathematical model was successfully verified with own measurements of frictional head loss in vertical coarse - dispersive slurry flow, named: ‘sand-water’, ‘polystyrene-water’ and ‘pvc-water’, , . The mathematical model can predict ‘particles-wall’ shear stress, pressure drop and friction factor for coarse-dispersive turbulent slurry flow in a pipe, . The aim of the paper is to present qualitative and quantitative dependence of solid particle diameter, solid particle density, solid concentration, and Reynolds number for carrier liquid phase on the ‘particles-wall’ shear stress. It is demonstrated that the solid particle diameter plays crucial role in its dependence on the ‘particles-wall’ shear stress. It was proved that in particular flow conditions the ‘particles-wall’ shear stress is much higher compared to the carrier liquid wall shear stress.
Mechanical properties of the pipeline samples that had been cut in annular and axial directions were investigated. The methodology of modeling and calculation of the real stress-strain state was described. The stable state during in the deformation process was defined. The results of the experimental researches were used as a test variant during examination of pipe strength.
Biological control of plant diseases is strongly emerging as an effective alternative to the use of chemical pesticides and fungicides. Stress tolerance is an important attribute in the selection of bacteria for the development of microbial inoculants. Fourteen salt-tolerant bacteria showing different morphological features isolated from the rhizosphere of maize were evaluated for different plant growth-promoting activities. All isolates showed auxin production ranging from 5 to 24 μg ⋅ ml–1 after 48 h incubation in tryptophan supplemented media. Phosphate solubilization ranged from 15 to 419 μg ⋅ ml–1. 1-aminocycloproprane- 1-carboxylate (ACC) deaminase activity was shown by 6 isolates, ammonia production by 9 isolates, siderophore production by 8 isolates while HCN production by 4 isolates. Four bacterial isolates with all plant growth-promoting properties also showed strong antagonistic activities against Fusarium oxysporum, F. verticillioides, Curvularia lunata and Alternaria alternata and abiotic stress tolerance against salinity, temperature, pH and calcium salts. Two selected bacterial isolates significantly enhanced the growth of pea and maize test plants under greenhouse conditions. The bacterial isolate M1B2, which showed the highest growth promotion of test plants, was identified as Bacillus sp. based on phenotypic and 16S rDNA gene sequencing. The results indicated that Bacillus sp. M1B2 is a potential candidate for the development of microbial inoculants in stressful environments.
This paper concerns the possibility of use the Jiles-Atherton extended model to describe the magnetic characteristics of construction steel St3 under mechanical stress. Results of the modelling utilizing extended Jiles-Atherton model are consistent with results of experimental measurements for magnetic hysteresis loops B(H). Material stress state determination by using non-destructive, magnetic properties based on testing techniques is an especially important problem.
Plants adapt to extremely low temperatures in polar regions by maximizing their photosynthetic efficiency and accumulating cryoprotective and osmoprotective compounds. Flowering plants of the family Poaceae growing in the Arctic and in the Antarctic were investigated. Their responses to cold stress were analyzed under laboratory conditions. Samples were collected after 24 h and 48 h of cold treatment. Quantitative and qualitative changes of sugars are found among different species, but they can differ within a genus of the family Poaceae. The values of the investigated parameters in Poa annua differed considerably depending to the biogeographic origin of plants. At the beginning of the experiment, Antarctic plants were acclimatized in greenhouse characterized by significantly higher content of sugars, including storage reserves, sucrose and starch, but lower total protein content. After 24 h of exposure to cold stress, much smaller changes in the examined parameters were noted in Antarctic plants than in locally grown specimens. Total sugar content and sucrose, starch and glucose levels were nearly constant in P. annua, but they varied significantly. Those changes are responsible for the high adaptability of P. annua to survive and develop in highly unsupportive environments and colonize new regions.