Background: Equine sarcoids are the most common neoplasms in horses. Bovine papilloma- virus type 1 (BPV-1) is the main viral type identified in equine sarcoids in Europe. Objective: The aim of the present study was to genetically evaluate BPV types based on DNA analyses of the CDS of the L1 gene. The presence of BPV DNA was confirmed by Degenerate Oligonucleotide-Primed Polymerase Chain Reaction (DOP PCR) with FAP59/FAP64 consensus primers. Results: The DNA was detected in 21/40 (52.5%) of clinically diagnosed sarcoids. More than half of 14 isolates (66.7%) shared 100% homology with BPV-1 Deltapapillomavirus 4 isolate 09 asi UK (Acc. No. MF384289) and 99% nucleotide identity with BPV-1 isolate EqSarc1 (Acc. No. JX678969). A comparison with BPV-1 isolate EqSarc1 revealed one silent mutation in C5827T which did not change the aminoacid codon. The remaining 6 isolates (28.6%) shared 100% nucleotide identity with the BPV-1 (Acc. No. X02346) “wild type” isolate, and 1 isolate (4.8%) demonstrated 99% nucleotide identity with BPV-2 (Acc. No. M20219). Conclusions: Variants of BPV-1 isolate EqSarc1 (Acc. No. JX678969) constitute the most prevalent type of BPV-1 in Polish horses.
The purpose of the study was to analyze a part of the nucleotide sequences of ystB gene Y. enterocolitica strains isolated from wild animals. The material for the study consists of 30 Y. enterocolitica biotype 1A strains obtained from different wild animal species and belonging to different genotypes. Phylogenetic analysis of ystB nucleotide sequences belonging to four regular genotypes G1, G2, G3, G4 and to five groups of variations V1, V2, V3, V4, V5 revealed significant differences of Y. enterocolitica strains isolated from wild animals. The most phylogenetically distant were strains belonging to V5.
The paper addresses the effect of a compost prepared from tobacco wastes with an admixture of bark and straw on the enzymatic activity and certain chemical properties of a grey-brown podzolic soil amended with that compost. The study was conducted under the conditions of a pot experiment in which the soil material was collected from the surface horizon of the grey-brown podzolic soil. The effect of the application of the compost was compared with soil without such amendment. The test plant was maize cv. Kosmo 230. Fertilisation of the light soil with the compost studied caused changes in the enzymatic activity of the soil that were related both to the dose of the compost and to the kind of enzyme studied. With increase in the dose of the compost there was an increase in dehydrogenase activity (highest dose) and a signiﬁcant decrease in the activity of acid phosphatase. Moreover, it was observed that tobacco compost was a signiﬁcant source that enriched the light soil in organic matter, total nitrogen, and available forms of phosphorus, magnesium and potassium, which was evident in increased yields of maize grown as the test plant. Signiﬁcant correlations were also demonstrated between a majority of the biochemical and chemical parameters, which indicates that those parameters characterise well the biological properties of a grey-brown podzolic soil amended with tobacco compost.
The Bulletin of the Polish Academy of Sciences: Technical Sciences (Bull.Pol. Ac.: Tech.) is published bimonthly by the Division IV Engineering Sciences of the Polish Academy of Sciences, since the beginning of the existence of the PAS in 1952. The journal is peer‐reviewed and is published both in printed and electronic form. It is established for the publication of original high quality papers from multidisciplinary Engineering sciences with the following topics preferred: Artificial and Computational Intelligence, Biomedical Engineering and Biotechnology, Civil Engineering, Control, Informatics and Robotics, Electronics, Telecommunication and Optoelectronics, Mechanical and Aeronautical Engineering, Thermodynamics, Material Science and Nanotechnology, Power Systems and Power Electronics. Journal Metrics: JCR Impact Factor 2018: 1.361, 5 Year Impact Factor: 1.323, SCImago Journal Rank (SJR) 2017: 0.319, Source Normalized Impact per Paper (SNIP) 2017: 1.005, CiteScore 2017: 1.27, The Polish Ministry of Science and Higher Education 2017: 25 points. Abbreviations/Acronym: Journal citation: Bull. Pol. Ac.: Tech., ISO: Bull. Pol. Acad. Sci.-Tech. Sci., JCR Abbrev: B POL ACAD SCI-TECH Acronym in the Editorial System: BPASTS.
The quarrying industry is changing the local landscape, forming deep open pits and spoil heaps in close proximity to them, especially lignite mines. The impact can include toxic soil material (low pH, heavy metals, oxidations etc.) which is the basis for further reclamation and afforestation. Forests that stand on spoil heaps have very different growth conditions because of the relief (slope, aspect, wind and rainfall shadows, supply of solar energy, etc.) and type of soil that is deposited. Airborne laser scanning (ALS) technology deliver point clouds (XYZ) and derivatives as raster height models (DTM, DSM, nDSM=CHM) which allow the reception of selected 2D and 3D forest parameters (e.g. height, base of the crown, cover, density, volume, biomass, etc). The automation of ALS point cloud processing and integrating the results into GIS helps forest managers to take appropriate decisions on silvicultural treatments in areas with failed plantations (toxic soil, droughts on south-facing slopes; landslides, etc.) or as regular maintenance. The ISOK country-wide project ongoing in Poland will soon deliver ALS point cloud data which can be successfully used for the monitoring and management of many thousands of hectares of destroyed post-industrial areas which according to the law, have to be afforested and transferred back to the State Forest.
Abstract The objective of the study was to assess the influence of methyl jasmonate (MJ) vapors on accumulation of 2-phenylethylamine (PEA), phenylacetic acid (PAA) and 2-phenylethanol (PE) in leaves and roots of maize (Zea mays L. subsp. mays, saccharata group, cv. Złota Karłowa) seedlings. Furthermore, we analyzed the expression patterns of eight genes (ADH1, ADH2, AO2, CAO, PDC1, PDC2, PTA and LOX, encoding alcohol dehydrogenase 1 and 2, primary amine oxidase, aldehyde oxidase 2, phenylalanine decarboxylase 1 and 2, phenylalanine (histidine) transaminase and lipoxygenase, respectively) involved in biosynthesis and turnover of PEA in maize tissues. In addition, the effect of MJ application on fresh biomass and growth of the tested seedlings was recorded. One-day MJ exposure increased the fresh weight of aerial parts and roots of Z. mays seedlings, whereas the opposite tendency occurred after 4-day of MJ treatment. One-day application of MJ resulted in an increase in the length of roots and its fluctuations in the aerial parts of maize plants, but extended exposure declined the growth of both parts of the seedlings. Methyl jasmonate elicitation caused various changes in the contents of PEA, PAA and PE in the maize seedlings. MJ treatments led to high upregulation of most genes, with the exception of three genes (i.e., ADH1, ADH2 and AO2) whose expression was downregulated after a 4-day exposure.