Early recognition of altered lactate levels is considered a useful prognostic indicator in dis- ease detection for both human beings and animals. It is reasonable therefore to hypothesize that a portable, point of care (POC) spectrophotometric device for analysis of lactate levels, may have an application for field veterinarians across a range of conditions and diagnostic procedures. In this study, a total of 72 cattle in the transition period underwent POC spectrophotometric lactate measurement with a portable device (The Vet Photometer) in the field, with a small portion of blood used for comparative ELISA evaluation. Lactate measurements were compared using a of Passing-Bablok regression analysis and Bland-Altman plots. The Vet Photometer lactate mea- surement results were in agreement with those generated by the ELISA method. Values for the agreement were derived, in a 95% CI between -1.3 and 0.99, and a positive correlation (r=0.71) between the two measurements. The equation y= 0.68x + 0.60 was achieved using a Pass- ing-Bablok regression analysis. There were no statistical differences in mean values between the measurement methods. In conclusion, a novel veterinary POC spectrophotometric device “Vet Photometer” is an accurate device for evaluation of lactate levels in healthy transition cows.
104 algal taxa (31 blue-green algae, 48 diatoms and 25 green algae) were identified from 18 stands of tundra soils in the Kaffiöyra Plain (Oscar II Land, NW Spitsbergen). Basing on numerical analysis by the reciprocal averaging method and on hierarchic classification based on ."distinguishing species", two groups of stands were distinguished: moist and wet ones characterized by diatoms, and dry and drying ones characterized by blue-green algae.
In individual dogs, despite good quality of raw sperm, some parameters are significantly changed after thawing, which cannot be predicted. We therefore investigated whether motility parameters objectively obtained by CASA, membrane integrity (MI), cell morphology or a combination are suitable to improve the prediction of bad post-thaw quality. For this purpose 250 sperm analysis protocols from 141 healthy stud dogs, all patients introduced for sperm cryopreservation, were evaluated and a Classification and Regression Tree (CART) -analysis performed. The sperm was routinely collected, analysed, and frozen by using a modified Uppsala system. After thawing, data were routinely examined by using CASA, fluorescent microscopy for membrane integrity (MI) and Hancock’s fixation for evaluation of cell morphology. Samples were sorted by post-thaw progressive motility (P) in good (P > / = 50%, n=135) and bad freezers (P<50%, n=115). Among bad freezers, 73.9% showed in addition post-thaw total morphological abberations of >40% and/or MI <50%. Bad freezers were significantly older than good freezers (p<0.05). Progressive motility (P), velocity curvilinear (VCL), mean coefficient (STR), and linear coefficient (LIN) were potential predictors for post-thaw sperm quality since specifity was best (85.8%) and sensitivity (75.4 %) and accuracy (80.4 %) good. For these objectively measured raw sperm parameters, cut-off values were calculated allowing prediction of bad post-thaw results with high accuracy: P = 83.1 % VCL = 161.3 µm/sec, STR = 0.83 %, and LIN = 0.48 %. Raw sperm samples with values below these cut off values will have below average post-thaw quality with a probability of 85.8%. We conclude that VCL, P, STR and LIN are potential predictors of the outcome of sperm cryopreservation, when combined.
The presence of lipopolysaccharide (LPS) in blood induces an inflammatory response which leads to multiple organ dysfunction and numerous metabolic disorders. Uncontrolled, improper or late intervention may lead to tissue hypoxia, anaerobic glycolysis and a disturbance in the acid -base balance. The effects of LPS-induced toxemia on biological and immunological markers were well studied. However, parameters such as base excess, ions, and acid-base balance were not fully investigated. Therefore, the objective of this study was to examine these blood parameters collectively in LPS-induced inflammatory toxemia in rat’s model. After induction of toxemia by injecting LPS at a rate of 5 mg/kg body weight intravenously, blood was collected from the tail vein of twenty rats and immediately analyzed. After 24 hours, the animals were sacrificed and the blood was collected from the caudal vena cava. The results revealed that the levels of pH, bicarbonate, partial pressure of oxygen, oxygen saturation, Alveolar oxygen, hemoglobin, hematocrit, magnesium (Mg2+), and calcium (Ca2+) were significantly decreased. On the other side, the levels of Base excess blood, Base excess extracellular fluid, partial pressure of carbon dioxide, lactate, Ca2+/Mg2+, potassium, and chloride were significantly increased compared to those found pre toxemia induction. However, sodium level showed no significant change. In conclusion, Acute LPS-toxemia model disturbs acid-base balance, blood gases, and ions. These parameters can be used to monitor human and animal toxemic inflammatory response induced by bacterial LPS conditions to assist in the management of the diagnosed cases.
The Blue Dyke and Jardine Peak are subvertical hypabyssal intrusions cutting a stratiform volcanic sequence in the Admiralty Bay area on King George Island (South Shetlands, Antarctica ). The rocks are porphyritic, crystal-rich basaltic andesites. Tiny zircon crystals were used for single grain SHRIMP U-Pb dating. The mean ages calculated for the zircon populations from both intrusions indicates Late Oligocene (Chattian) formations. Zircon grains from the Blue Dyke gave the mean age of 27.9±0.3 Ma, whereas those from the Jardine Peak are slightly younger displaying the mean age of 25.4 ± 0.4 Ma: a Late Oligocene (Chattian) crystallization age the inferred of both these intrusions. These are much younger than previous Eocene K-Ar and Ar-Ar ages for such rocks and suggest that formation of the King George Island intrusions can be related to tectonic processes that accompanied the opening of the Drake Passage.
The results of the detailed seismoacoustic profilling (CSP, boomar) are presented. The investigation has been carried out in February 1985 and 1988 during two Geodynamical Expeditions organized by the Institute of Geophysics of the Polish Academy of Sciences. The boomar penetration of the caldera floor went down to 150 msec. Four seismoacoustic units of volcanic formations have been determined. The unit A corresponds to pre-caldera series and occurred only in the border part of the flooded caldera. The unit contains mainly pyroclastic rocks (consolidated agglomerates and tuffs) and probably some intercalations of lavas. The units B, C and D fill up the caldera bottom and correspond to post-caldera series. The units are composed of pyroclastic rocks, containing also materials redeposited by lahars, glaciers, landwaters and by wind. The units C and D (the youngest one) were certainly deposited under water. All the units are cut by numerous faults, vents and other types of intrusions. The larger faults, en echelon type, are situated around the bottom and form a ring-fracture. Caldera was formed by succesive stages of collapsing. This process is not finished yet and volcanic activity is still alive (especially in the western part of the flooded caldera).
On the ground of results obtained by the seismoacoustic profiling carried out in 1985 and primary examination of core samples the following main seismoacoustic units are distinguished and characterized: unit A — bedrock, unit B — till and/or compacted glaciomarine deposit, unit C — glaciomarine ice-front deposit, unit D — glaciomarine mud. These results enabled to present the distribution of seismoacoustic units along the fiord and its extension on the shelf, as well as to determine a relation of bottom structures to Late Vistulian(?) deglaciation and the action of Holocene tributary glaciers, probably during the Little Ice Age. The position of marginal structures corresponding to local retreat stages of the glacier front is also presented.
A sea floor investigation was performed in the fiord of Hornsund by means of the seismoacoustic profiling, echosounding and core sampling. The main seismoacoustic sea floor units were recognized (the methods used according to Kowalewski et al. 1987a) and characterized on the basis of their relations to geomorphology and geological evolution. The bathymetrical sketch and the resulting geomorphological description of the bottom were prepared. The surface of the sea bottom and the surface of the bedrock displayed an irregular high relief with large sills dividing the fiord sea floor into several basins. Four main types of the sills were distinguished: burried sills, accumulative sills, rock sills and rock-accumulative sills. Within the internal Basins I and II there were thick (up to 170 m) covers of the glaciomarine ice-front deposit with changing thin ( 1 -5 m) blanket of the glaciomarine muds at the bottom surface. The Basin III had a cover of the glacial and glaciomarine deposits of variable lithology, genesis and age. The most external Basin IV had a cover o f glaciomarine muds up to 4 0 - 5 0 m thick, deposited on the tills. Four main glacial episodes were recognized, most probably referring to the stadials of Lisbetdalen, Slaklidalen, Revdalen and to the Little Ice Age.
The aim of this study was to determine the effects of factors such as sex, eye-side and diurnal variation on intraocular pressure (IOP) values measured by Tono-Pen Vet® in healthy calves. Twenty four (12 males, 12 females) calves were used in this study. IOP measurements were per- formed in the morning (6:00 a.m.) and at night (8:00 p.m). Average IOP values in all calves were measured as 17.67±2.64 mmHg in the morning and 15.52±2.05 at night and the difference between these two time points was found to be statistically significant (p<0.0001). Average IOP values were measured as 16.04±2.82 mmHg in males and 17.15±2.23 mmHg in females with a statistically significant (p<0.05=0.023) difference between them. Average IOP values were 16.81±2.85 mmHg in right eyes and 16.37±2.23 mmHg in left eyes and the difference between these values were statistically insignificant (p>0.05). At the end of study, overall average IOP in all calves was measured as 16.59±2.59 mmHg. The present data showed a significant difference in terms of sex and diurnal variations and a non-significant difference in eye-side. In addition, this study is the first research article in which the intraocular pressure in calves was measured by Tono-Pen Vet®.
US A356 and US 413 cast aluminium alloys shrinkage characteristic have been discussed in the present study. Specific volume reduction leads to shrinkage in castings and it can be envisaged as a casting defect. Finite difference based casting process simulation software has been used to study the shrinkage characteristic and it is quantified using mathematical formulae. The three dimensional model of the shrinkage defect has been constructed using CAD application software. Shrinkage characteristic has also been quantified through experimental validation studies and compared well with casting process simulation. Shrinkage characteristic study and control is essential for producing defect free castings. Influence of casting shape on the shrinkage characteristic has been studied in this paper.
In the paper, a feedforward linearization method for differential-pair operational transconductance ampliﬁer (OTA) is discussed. The proposed technique is developed using simple differential pair transconductors and linear reference resistor. The concept leads not only to very efficient linearization ofa transfer characteristic oft he OTA but also others the possibility of effﬀective phase compensation. Due to this, the circuit can be used in applications requiring precise phase response (e.g. ﬁlters). SPICE simulations show that for the circuit working with a ±1.25V power supply, total harmonic distortion (THD) at 0.8Vpp is less then 0.1% in comparison to 10.2% without linearization. Moreover, the input voltage range ofline ar operation is increased. Power consumption oft he overall circuit is 0.94mW. The 3rd order elliptic ﬁlter example has been designed and simulated. It turns out that the proposed compensation scheme signiﬁcantly improves the performance of the ﬁlter at higher frequencies.
In the paper, a general topology of continuous-time Active-RC filter is presented. The model includes all possible Active-RC filter structures as particular cases and allows us to analyze them using a unified algebraic formalism. This makes it suitable for use in computeraided analysis and design of Active-RC filters. By its construction, the model takes into account the finite DC gain and the finite bandwidth as well as non-zero output resistance of operational amplifiers. Filters with ideal OPAMPs can be treated as particular cases. Sensitivity and noise analysis of Active-RC filters is also performed in the proposed general setting. The correctness of the model is verified by comparison with SPICE simulation.
In this study, we propose a possible way of obtaining reproductive and respiratory syndrome virus (PRRSV) free offspring from genetically valuable lines of Přeštice black-pied boars com- ming from PRRSV-positive pig breeding herds with the use of artificial insemination (AI). The ejaculates were collected from 4 different lines of boars. Samples of fresh semen were not detected with the virus and 12 sows were inseminated. Blood samples of sows and their offspring were repeatedly tested for the virus but the results were negative. We managed in this way to maintain the endangered population of this breed and obtain PRRSV-free offspring.
Several human studies have reported that capsaicin has anti-pruritic effects. Moreover, sever- al concentrations of topical capsaicin have been used to alleviate itch. The aim of this study was to investigate the anti-pruritic effect of capsaicin against histamine-induced pruritus compared with that of topical steroid or vehicle in 15 healthy beagles. Fifteen dogs were divided into three groups (n = 5 each), and treated topically with one of the following on the left side of the neck: capsaicin, positive control (steroid), or negative control (vehicle). Each treatment was performed twice daily for 8 days. All dogs were injected with histamine intradermally before treatment and on the 2nd, 4th, 6th, and 8th days of the treatment to evoke itch. Pruritus, wheal, and erythema intensity were assessed at each evaluation; cutaneous temperature was also recorded. On the final day, skin biopsy was conducted for histopathological evaluation for all dogs. The severity of pruritus was lesser in the capsaicin-treated group compared with the negative control group on day 8 (p<0.05). In the capsaicin and steroid groups, wheal size, erythema index, and cutaneous temperature also decreased compared with pretreatment. Histopathological evaluation showed that the capsaicin-treated group had a higher number of inflammatory cells in the dermis com- pared to the vehicle control group; however, the steroid-treated group showed less severe inflam- matory reactions than the vehicle control group. These results suggest that capsaicin cannot reduce inflammation but may play a helpful role in reducing pruritus in dogs.
A clinical trial was performed to evaluate the therapeutic efficacy of osaterone acetate (OSA) in the treatment of benign prostatic hyperplasia (BPH) in dogs. Osaterone acetate (Ypozane, Virbac) was administered orally at a dose of 0.25 mg/kg body weight once a day for seven days to 23 dogs with BPH. During the 28-day trial, the dogs were monitored five times for their clinical signs and prostate volume. The OSA treatment promoted rapid reduction of clinical scores to 73.2% on day 7 and to 5.9% on day 28 (p<0.05). Osaterone acetate induced the complete clinical remission in approximately 83.0% of the dogs on day 28. The prostate volume regressed to 64.3% of the pretreatment volume after two weeks of the treatment (p<0.05) and to 54.7% at the end of the trial (p<0.05). In conclusion, OSA quickly reduced clinical signs and volume of the prostate glands in dogs with BPH.
The paper presents results of investigations of bottom sediments in Hornsund, Wijdefjorden and Isfjorden as well as of the shelf around the Bjornoya. carried out in 1982—1985 by a continuous seismic profiling. Geophysic structures and bottom sediments on the bedrock to a depth of 170 ms have been recognized, particularly in the Hornsund region. The following seismoacoustic units have been distinguished: unit A — bedrock, unit В — till and/or compacted glaciogenic deposit, unit С — glaciomarine ice-front deposit, unit D — glaciomarine mud. These results allowed to present a model of glaciomarine sedimentation in a fiord, fed by warm tidewater glaciers.
The objective of this study was to evaluate the effect of a second prostaglandin F2α (PGF2α) treatment during Ovsynch on luteal regression and fertility in dairy cows, compared with standard Ovsynch. The study was conducted on 111 Holstein Friesian multiparous cows on commercial dairy farm. The cows in the experimental group (n=48) received two treatments of PGF2α 24 hours apart during Ovsynch. The cows in the control group (n=63) were synchronized with standard Ovsynch. To assess the progesterone (P4) concentration blood samples were collected at the day of PGF2α treatment and at the 2nd GnRH treatment. Pregnancy was evaluated by ultrasound examination 37-40 days after timed artificial insemination (TAI) by ultrasound. Cows diagnosed pregnant were re-examined between days 70-80 after TAI. The percentage of cows with complete corpus luteum (CL) regression (P4<0.5 ng/ml at the time of the 2nd GnRH treatment) was 89.6 % after two PGF2α treatments and 88.9 % after one PGF2α treatment. There were no statistically significant differences (p>0.05) in the pregnancies per artificial insemination (P/AI) between the experimental and control group (P/AI). However, the pregnancy loss rate was lower in cows receiving two PGF2α treatments than in the control animals (0.0 % vs. 6.4 %; p<0.05). In conclusion, the second PGF2α treatment during Ovsynch protocol had no significant effect on CL regression and P/AI in dairy cows. The pregnancy losses until days 75-80 after TAI were significantly lower after two PGF2α treatments than after one PGF2α treatment.
The study was conducted on 26 male, 30 days-old goats, separated from their mothers, divided into two equal groups: I - control and II - experimental, consisting of 13 animals each. All animals were fed with milk replacer, experimental group received additionally 50 g/kg body weight, additive of HMB, for 60 days. The following features were analyzed: body weight, daily increases of body weight, as well as hematological and biochemical blood features. Differences in body weight were found, between experimental and control group, after 60 days of experiment 0.57 kg (p≤0.01). The daily weight gain of experimental animals was higher in comparison with control group. Significant differences were also noted in results of hematological and biochemical blood parameters. Experimental animals showed a higher level of red blood cells as well as number of lymphocytes in comparison with the control group, (p≤0.01).Significant changes were also observed in the level of triglycerides, inorganic phosphorus and protein between both groups. The acid-base balance parameters and ionogram, showed a higher pH level (p≤0.05) HCO – act., HCO – std., BE, ctCO , O sat, K+, Cl– (p≤0.01), while the anion gap (AG) and Na+ were significantly lower in control group (p≤0.01).