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Biochemical Oxygen Demand (BOD) is an important factor used to measure water pollution. This article reviews recent developments of microbial biosensors with respect to their applications for low BOD estimation. Four main methods to measure BOD using a biosensor are described: microbial fuel cells, optical methods, oxygen electrode based methods and mediator-based methods. Each of them is based on different principles, thus a different approach is required to improve the limit of detection. A proper choice of microorganisms used in the biosensor construction and/or sample pre-treatment processes is also essential to improve the BOD lower detection limit.
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