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Abstrakt

This paper proposes a novel method for digital image watermarking, in which watermarks are embedded in the domain of fast para-metric transforms based on known spread spectrum approaches. Fast parametric transforms have the ability to adapt the forms of base vectors, which enables automatic selection of the domain of watermarking in relation to the pair: a marked image – a watermarking attack. The process of adapting the forms of fast parametric transforms is carried out with aid of the classical genetic algorithm with the fitting function based on the known measure of separability of watermarks. The effectiveness of the proposed method has been verified experimentally on the basis of the images of two classes, i.e. natural images and technical diagrams. The results taking into account both the efficiency of watermark embedding and the generated distortions in the marked images are summarized in tables and accompanied by an appropriate commentary.
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Abstrakt

Animals as a source of organs and tissues for xenotransplantation could become a backup solution for the growing shortage of human donors. The presence of human xenoreactive anti- bodies directed against Galα1,3Gal antigens on the cell surface of a pig donor triggers the activa- tion of the complement leading to a hyperacute reaction. The development of genetic engineer- ing techniques has enabled the modification of genomes by knocking in and/or knocking out genes. In this paper, we report the generation of modified pigs with ZFN mediated disruption of the GGTA1 gene encoding the enzyme responsible for synthesis of Galα1,3Gal antigens. ZFN plasmids designed to target the exon 9 region of the pig GGTA1 gene encoding the catalytic domain were injected into the pronuclei of fertilized egg cells. Among 107 piglets of the F0 gene- ration analyzed, one female with 9-nt deletion in exon 9 of the GGTA1 gene was found. 13 of 33 piglets of the F1 generation represented the +/- GGTA1 genotype and 2 of 13 F2 piglets repre- sented the -/- GGTA1 genotype. No changes in the animals’ behavior, phenotype or karyotype were observed. Analysis confirmed heredity of the trait in all animals. A complex functional analysis of the modified animals, including flow cytometry, human serum cytotoxicity test and immunohistochemical detection, was performed to estimate the phenotype effect of genetic modification and this indicated an efficient GGTA1 knock-out in modified pigs.
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